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On-line enzymatic and nonenzymatic conversion of heroin (10 μM) to 6-acetylmorphine and morphine in columns containing approximately 50 iHLC organoids (sample) and columns containing no organoids (control). Graphs show average areas of heroin and metabolites normalized to the average total area of heroin, 6-acetylmorphine, and morphine (area-% of avg. total area of heroin, 6-acetylmorphine, and morphine). The average total areas of heroin, 6-acetylmorphine, and morphine are based on three runs performed on different days (12 h experiments) and columns with organoids from the WTC-11 cell line (error bars: standard deviation, n = 3).

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Figure Lengend Snippet: On-line enzymatic and nonenzymatic conversion of heroin (10 μM) to 6-acetylmorphine and morphine in columns containing approximately 50 iHLC organoids (sample) and columns containing no organoids (control). Graphs show average areas of heroin and metabolites normalized to the average total area of heroin, 6-acetylmorphine, and morphine (area-% of avg. total area of heroin, 6-acetylmorphine, and morphine). The average total areas of heroin, 6-acetylmorphine, and morphine are based on three runs performed on different days (12 h experiments) and columns with organoids from the WTC-11 cell line (error bars: standard deviation, n = 3).

Article Snippet: Heroin-d9, 6-acetylmorphine -d6, and morphine-d3 (used for heroin stability experiments only) were purchased from Cerilliant (Austin, TX).

Techniques: Standard Deviation